تاًثیر متقابل سرب و اسپرمیدین بر روی برخی شاخص های فیزیولوژیکی و بیوشیمیایی گیاه مریم گلی ( Salvia officinalis L.)

Production of heavy metals by human activity is caused soil contaminations. The polyamines are used to reduction of heavy metal effects. In this study, the effects of lead and spermidine on Salvia officinalis L. were tested. A factorial experiment was conducted in a completely randomized design with three replications. Lead nitrate at concentrations of 0, 125, 250 and 1000 micro molar and spermidine at a concentration of 0 and 1 mM was used. Plant height, dry and fresh weight, catalase and polyphenol oxidase activity, antioxidant capacity and total phenols contents was measured. The utilization of lead nitrate increased the length, dry weight, catalase activity except at a concentration of 125 micro molar and poly phenol oxidase activity. Antioxidant capacity did not change except in a concentration of 1000 micro molar. Total phenols content increased in 250 micro molar concentrations and the other treatments were not much difference. Use of spermidine and lead increased the length in a concentration of 500 and 1000 micro-molar. Dry weights increased in all treatments. The maximum catalase activity was observed in a concentration of 500 micro-molar lead and spermidine. The minimum activity was seen in a concentration of 250 micro-molar with spermidine. Poly phenol oxidase activity increased just in a concentration of 250 micro-molar lead with spermidine. The total phenols and antioxidant capacity decreased under 500 micro-molar of lead and spermidine. Addition of spermidine under lead stress increased plant length and weight and in low concentrations of lead total phenols increased